Monday, April 5, 2010

Techniques, Sapprophyte

Sexual stages of 3 classes:

Basidiomycetes
brown basidiospore


Zygomycetes
dark colored zygospore formed from fusion of 2 aseptate hyphae


Ascomycetes
the 2 celled ascospores are produced in a sac like ascus

Dermatophytes and other fungi that causes superficial infections:
limited to keratinized tissues. ie. skin, hair, nails
dermatophyte infection determined by observing skin scrapping in KOH wet mount
has macroconidia & microconidia


Microsporum canis
spindle-shaped, thick walled macroconidia.
has up to 15 septa.

Microsporum gypseum
thin wall
usually 4-6 septa

Epidermophyton floccosum
club-shaped macroconidia
2-3 septa
no microconidia


Tricophyton mentagrophytes
cigar-shaped
thin walled macroconidia
abundant
coiled hyphae
hair perforation positive


Tricophyton rubrum
cigar-shaped macroconidia
thin walled microconidia
Dermatophyte
anthropophillic (attracted to man*), zoophillic, geophillic [as pop. decrease, lose ability to mate]

Saprophytes: Organisms that live on dead organic matter


Aspergillus
chained conidia on phialides arise from swollen vesicle
Exogenous opportunistic fungus.


Scopulariopsis spp.
geicohands-like conidiophore
large, rough, annelloconidia (produced by annelides)


Penicillium
chains of conidia from branched conidiaphore
brush like, longer than Aspergillus

Penicillium marnefei
severe biohazard
intracellular (Histo too!!)
dimorphic
haploid, no sexual stage
most infected by HIV positive patients

Disease:(by inhalation)
once infected, cannot be cured
effect immune surpress individuals (ie. HIV)

Disribution:
Southeast Asia, nothern Thailand
Diagnose:
Culture, PCR, Serological test




Rhizopus
non-septate, wide hyphae
sporangia filled with sporangiospores
has rhizoids at base of sporangiophore
Rndogenous opportunistic fungi

Mucor
septate hyphae
sporangiospores in sporangia.

Alternaria
dematiaceous (dark colored)
club-shaped conidia

1 comment:

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